Improved site-specific recombinase-based method to produce selectable marker- and vector-backbone-free transgenic cells
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چکیده
منابع مشابه
Improved site-specific recombinase-based method to produce selectable marker- and vector-backbone-free transgenic cells
PhiC31 integrase-mediated gene delivery has been extensively used in gene therapy and animal transgenesis. However, random integration events are observed in phiC31-mediated integration in different types of mammalian cells; as a result, the efficiencies of pseudo attP site integration and evaluation of site-specific integration are compromised. To improve this system, we used an attB-TK fusion...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2014
ISSN: 2045-2322
DOI: 10.1038/srep04240